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BIOTECHNOLOGY

Peroxynitrite: Relevance for Food Safety and Novel Electrochemical Detection
Peroxynitrite:
Relevance for Food Safety and Novel Electrochemical Detection
 



PEROXYNITRITE    

About project

  • Project title: Peroxynitrite: Relevance for Food Safety and Novel Electrochemical Detection
  • Project code: PN-II-ID-PCE-2011-3-1076
  • Contract no.: 184/2011
  • Project duration: 2011 - 2016 (62 months)
  • Project manager:
    • Dr. Eng. Serban PETEU (2011 - 2013)
    • Dr. Biochem. Mihaela (BADEA) DONI (2014 - 2015)
  • Contractor: National Research Institute for Research & Development in Chemistry and Petrochemistry - ICECHIM, Bucharest, ROMANIA
  • Financing authority: Executive Agency for Higher Education, Research, Development and Innovation Funding
  • Project webpages:

Project summary and objectives

Considering this toxicity potential of peroxynitrite formation in meat with impact for food safety, it is proposed herein to develop advanced tools to quantify the presence of peroxynitrite in food: meat, in real-time in situ. While there are several detection methodsavailable for peroxynitrite (e.g. oxidation of fluorescent probes, EPR spectroscopy, probe nitration, chemiluminescence, immun-histochemistry) these tend to be more elaborate and difficult to apply for real-time quantification,  due to their inherent complexity.  The electrochemical detection of peroxynitrite would be a simpler and more convenient technique.

Based on initial results and specific knowhow, the team final goals are to develop electrochemical ONOO- sensitive in situ methods, based on new/improved (metalloporphyrins and conjugated polymers) nanocomposies, for detection its dynamics in foods such as meat-fish tissue and use the results to better understand ONOO- implications for food quality and safety. Once perfected as analytical tools, the peroxynitrite microprobes could be studied for possible use in pharmacology and toxicology.



Project team

  • Dr. Serban PETEU - project manager (2011 - 2013)
  • Dr. Mihaela (BADEA) DONI - project manager (since 2014)
  • Dr. Florin OANCEA, Senior Scientist I
  • Dr. Ramona Marina COSEREA, CS
  • Eng. Raluca Ana-Maria OPREA, M.Sc. student
  • Dr. Mihaela CALUGAREANU, CS
  • Isabela DUMITRU, M.Sc. student
  • Chem. Ioana Silvia HOSU, M.Sc. student
  • Eng. Monica Adriana GRUMAZ, M.Sc. student
  • Biochem. Mariana POPESCU, Senior Scientist II
  • Ana Maria POPESCU, M.Sc. student
  • Dr. Maria-Luiza JECU, Senior Scientist I
  • Dr. Diana CONSTANTINESCU-ARUXANDEI, Research Assistant

Scientific reports

  1. 2011 Scientific Report
  2. 2012 Scientific Report
  3. 2013 Scientific Report
  4. 2014 Scientific Report
  5. Scientific Report for 2011 - 2014 (English)
  6. 2015 Scientific Report (Romanian)
  7. 2015 Scientific Report (English)
  8. 2016 Scientific Report (English)
  9. Scientific Report for the entire project (2011-2016)

Results

ISI Papers
  1. R.H. Perera, P.M. Peris, S.F. Peteu, M. Bayachou, Nitric oxide synthase loaded liposomes as a platform for nitric oxide delivery of NO deficient targets, Electroanalysis 24: 37-41, 2012.
  2. S.F. Peteu, T. Bose, M. Bayachou, Polymerized hemin for peroxynitrite electrocatalytic oxidation and detection, Analytica Chimica Acta, 780: 81-88, 2013.
  3. R. Oprea, S.F. Peteu, P. Subramanian, E. Pichonat, H. Happy, R. Boukherroub, S. Szunerits, High Peroxynitrite activity of hemin-functionalized reduced graphene oxide formed in an environmental friendly way, Analyst,138: 4345-4352, 2013.
  4. I.S. Hosu, Q. Wang, A. Vasilescu, S. F. Peteu, V. Raditoiu, S. Railian, V. Zaitsev, K. Turcheniuk, Q. Wang, M. Li, R. Boukherroub, S. Szunerits, "Cobalt phthalocyanine tetracarboxylic acid modified reduced graphene oxide: a sensitive matrix for the electrocatalytic detection of peroxynitrite and hydrogen peroxide", RSC Advances, 5, 1474-1484, 2015.
  5. I.S. Hosu, D. Constantinescu-Aruxandei, M.-L. Jecu, F. Oancea, M. Badea Doni, Peroxynitrite Sensor Based on a Screen Printed Carbon Electrode Modified with a Poly(2,6-dihydroxynaphthalene) Film, Sensors, 16(11), 1975 – 1990, 2016.
Books and chapters in books
  1. S.F. Peteu, M.S. Banihani, M.M. Gunesekera, P. Peiris, O.A. Sicuia, M. Bayachou, Peroxynitrite and nitroxidative stress: detection probes and micro-sensors. A case of a nanostructured catalytic film, Oxidative stress and antioxidants: diagnosis and therapy, pp 311-339, Edited by Andreescu & Hepel, American Chemical Society Symposium Series, ISBN 9780841226845, 2011.
  2. S.F. Peteu, S. Szunerits, A. Vasilescu, W. Knoll. Nanoscale architectures for smart biointerfaces: Advances and challenges. Nanofabrication, pp 63-98, Intech, Yoshitake, Edited by Masuda, ISBN 978-953-307-912-7, 2012.
  3. S.F. Peteu, S. Szunerits, Peroxynitrite Quantification Challenges and Recent Advances, Detection Challenges in Clinical Diagnostics, Royal Society of Chemistry, Cambridge, UK, Edited by Vadgama & Peteu, ISBN 978-1-84973-612-1, 2013.
  4. A. Vasilescu, A. Vezeanu, Y. Liu, I. Hosu, R. Worden, S. Peteu, Chapter 16: "Meat Freshness: Peroxynitrite's Oxidative Role, Its Natural Scavengers, and New Measuring Tools", pp. 303-332, in: "Instrumental Methods for the Analysis of Bioactive Molecules", ACS Books, 2015.
  5. A. Vasilescu, V. Dinca, M. Filipescu, L. Rusen, I.S. Hosu, R. Boukherroub, S. Szunerits, M. Dinescu, S.F. Peteu, Chapter 9: "Recent Approaches to Enhance the Selectivity of Peroxynitrite Detection", pp. 166 – 185, in: "Peroxynitrite Detection in Biological Media: Challenges and Advances", RSC Detection Science, Editors: S. Peteu, S. Szunerits, M. Bayachou, 2015.
  6. Andrei F. Danet, Mihaela Badea – Doni, Chapter 32: "Determination of Lipid Oxidation by Chemiluminescence Reagents", pp. 623-638, in: "Flow Injection Analysis of Food Additives", CRC Press, Francis & Taylor Group, Editors: Claudia Ruiz-Capillas, Leo M. L. Nollet, 2015.
  7. B. Bucur, I.S. Hosu, A. Vasilescu, Chapter: "Biosensors for characterization of bioactive compounds from wines", pp. 147-189(43) in: "Bioactive compounds: natural sources, physicochemical characterization, applications, Bentham Science Publishers, Editor: C.Apetrei
International Conferences
  1. S.F. Peteu, R. Coserea, F. Oancea, Electroactive Nano-materials and Their Potential for Precision Agriculture, Poster presentation, the 63rd Annual ISE Meeting, Prague, Czech Republic, 19-24 August, 2012.
  2. S.F. Peteu et al., Poster presentation, the RSE-SEE3, Bucharest, 13-17 May 2012.
  3. S.F. Peteu, R. Oprea, T. Bose, R. Boukherroub, S. Szunerits, M. Bayachou, Recent Advances in Peroxynitrite Detection, Oral presentation, the 63rd Annual Meeting of the International Society of Electrochemistry (ISE), Prague, Czech Republic, 19-24 August, 2012.
  4. S.F. Peteu et al., Oral presentation, the Third Regional Symposium on Electrochemistry - South-East Europe (RSE-SEE3), Bucharest, Romania, 13-17 May, 2012.
  5. S.F. Peteu et al., New peroxynitrite-sensitive microsensor and its potential to quantify the muscle protein oxidative dynamics, Oral Presentation accepted, the 246th ACS National Fall Meeting, 3-8 September, 2013.
  6. S.F. Peteu et al., An analytical chemistry challenge and recent advances: peroxynitrite quantification, Oral Presentation accepted, the 246th American Chemical Society (ACS) National Fall Meeting, Indianapolis Indiana, United States, 3-8 September 2013.
  7. S.F. Peteu et al., Graphene complex with metallo-macrocycles as electrocatalytic film created by matrix-assisted pulsed laser evaporation (MAPLE) and chemical sensing application, Oral Presentation accepted, the 246th ACS National Fall Meeting, 3-8 September 2013.
  8. I. S. Hosu, M. Badea-Doni, The electrocatalytic oxidation of peroxynitrite at a modified Co(II) phthalocyanine Glassy Carbon Electrode, Poster at New Trends on Sensing- Monitoring- Telediagnosis for Life Sciences, Brasov, Romania, 24-26 Iulie 2014.
  9. I. S. Hosu, M. Badea-Doni, Electrochemical detection of peroxynitrite on a cobalt phthalocyanine modified Screen-Printed Carbon Electrode, Poster at RO-ICAC'2014 (International Conference on Analytical Chemistry - Analytical Chemistry for a Better Life), Targoviste, Romania, 17-21 Septembrie 2014.
  10. S.O. Dima, I.S. Hosu, M. Badea-Doni, F. Oancea, T. Dobre, Acrylic copolymer-based sensors for the detection of hydrogen peroxide, Poster at RO-ICAC'2014 (International Conference on Analytical Chemistry - Analytical Chemistry for a Better Life), Targoviste, Romania, 17-21 Septembrie 2014.
  11. I.S. Hosu, M. Badea-Doni, Cobalt(II) phthalocyanine Screen Printed Carbon Electrode for studying the interaction between myoglobin and peroxynitrite, Oral Presentation at International Symposium PRIOCHEM (Prioritatile Chimiei pentru o Dezvoltare Durabila), Editia a-X-a, Bucharest, Romania, 30-31 Octombrie 2014.
  12. I.S. Hosu, M. Badea-Doni, Flow injection system for the electrochemical detection of peroxynitrite with a cobalt phthalocyanine modified screen printed carbon electrode, Flow Analysis XIII, Praga, Cehia, 5-10 July 2015
  13. I.S. Hosu, F.Oancea, M. Doni, Flow injection system for the electrochemical monitoring of peroxynitrite and its reaction with myoglobin, Priorities of Chemistry for a Sustainable Development - PRIOCHEM XI, Bucure?ti, România, 29-30 October 2015
  14. M. Badea Doni, M.-L. Arsene, M.-L. Jecu, Sensors based on screen printed electrodes for nitrite and peroxynitrite determination in meet, Invited lecture at International Workshop 'New methodologies and advanced nanomaterials for electrochemical biosensors', Bucharest, Romania, 20 May 2016
  15. M. Badea Doni, I.S. Hosu, D. Constantinescu-Aruxandei, M.-L. Arsene, F. Oancea, M.-L. Jecu, Flow injection system based on a modified screen printed electrode for peroxynitrite determination in meat extracts, Poster at RO-ICAC'16 - 3rd International Conference on Analytical Chemistry, Iasi, Romania, 28 – 31 August 2016
  16. M. Badea Doni, G. Petcu, M. Patrascu, M.-L. Arsene, V. Fruth, M-L Jecu, Screen printed carbon electrode modified with copper oxide nanoparticles for glucose determination, Poster at RO-ICAC'16 - 3rd International Conference on Analytical Chemistry, Iasi, Romania, 28 – 31 August 2016
  17. M. Badea Doni, I.S. Hosu, D. Constantinescu-Aruxandei, M.-L. Arsene, M.-L. Jecu, F. Oancea, Peroxynitrite sensor based on a modified screen printed carbon electrode, Poster at The 16th International Conference of Physical Chemistry "ROMPHYSCHEM", Galati, Romania, 21-24 September 2016
  18. I.S. Hosu, D. Constantinescu-Aruxandei, M.-L. Jecu, M.-L. Arsene, F. Oancea, M. Badea Doni, Screen printed carbon electrodes modified with cobalt phthalocyanine vs poly(2,6 – dihydroxynapthalene) for peroxynitrite determination, International Symposium 'Priorities of Chemistry for a Sustainable Development' - PRIOCHEM 12nd Edition, Bucharest, Romania, 27-28 October 2016
Patents
  1. PROCEDEU DE OBŢINERE ŞI DE UTILIZARE A UNUI MATERIAL ELECTROSENZITIV PENTRU DETERMINAREA SELECTIVĂ A PEROXINITRITULUI
    Mihaela Doni, Ioana Silvia Hosu, Maria-Luiza Jecu, Florin Oancea
    Patent application OSIM no.: A2015/00929 / 27.11.2015

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